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How Genetics Is Perfecting The Christmas Tree

12/21/2012

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The Fraser fir is an ideal Christmas tree, but it could always use improvement. One tree scientist is making them stronger and hardier.



With just a few days left until Christmas, my tree is barely holding on--to its needles, to its stiffness, and to its dignity. It was an early tree this year, the result of the earliest possible Thanksgiving, but it was more than that. There was something about this tree that made it dry out quickly, and make it less likely to keep its thin needles. It is a Fraser fir, Abies fraseriis, so this was a surprise to John Frampton.

Frampton, a professor in the department of Forestry and Environmental Resources at North Carolina State University, is the expert on Fraser firs, the most popular Christmas tree. Their conical shape, superb needle retention and sturdy boughs make them perfect for keeping both ornaments and tree bits off the floor. But Frampton is trying to make them even better, helping growers fight a worsening root fungus problem and to grow more trees more quickly.

As a result, Christmas trees of the future will be hybrids of different plant species, with parts borrowed and bred from the hardiest and most Christmasy species.

Fighting root rotA Christmas tree spends six to 10 years in the field before it’s cut, and trees usually aren’t planted in tree plantations until they’re at least two or three--meaning my Christmas tree was born when I was still in my teens. It turns out those early years of the tree’s life were its most difficult.

Fraser firs have no immunity against phytophthora, a genus of damaging molds that cause plant roots to rot. Its Greek name means “plant destroyer,” and it can decimate crops and almost any other type of plant--a phytophthora species caused the potato blight that sparked the Great Irish Famine. Frampton has tried to find resistant trees to breed, but to no avail.

“We grow seedlings in the greenhouse and we inoculate them to test their resistance; when we do that, we kill them all,” he said. “We’ve tested a very genetically diverse population several times, and every time we test it, we kill the trees.”

To make stronger trees, he has to perform a bit of vegetation vivisection. Frampton tested 32 of the world’s 50-odd true fir species, and found a Japanese tree called the Momi fir strongly resists phytophthora invasion. They make terrible Christmas trees, so Frampton teaches tree growers how to borrow their roots.

He buys Momi fir seedlings and cuts off the tree part, grafting Fraser fir branches into their root systems. Grafting is a common horticultural technique to essentially clone different plants, but in this case it’s making chimeric trees.

“We take a young seedling of Momi fir and cut the top off. Then we take a grafting knife, and make a vertical slit down the center. We take a Fraser fir branch and trim the base into a wedge, and we insert that into the Momi fir that we cut,” Frampton explained. The newly grafted tree is wrapped in special rubber bands and covered in wax to keep it from drying out.

“Then the area between the bark and the wood, the growing point, merges. It grows together for the two species, and they form one functioning tree. The top of the tree is Fraser fir, and everything below the graft union is still Momi fir.”

This is kind of expensive, however, and time-consuming. Tree growers would prefer seedlings that already have root-rot resistance, but maintain the ideal needle retention and pretty conical shape of Frasers. That’s where genetics comes in.

Breeding tougher treesWhen he was testing seedlings for root-rot resistance, Frampton went to Turkey and gathered cones from Nordmann fir trees, inoculating seedlings he grew in his greenhouse. Some of them can resist it, he found--activation of certain genes can control that resistance. That means Frampton and other growers can select trees that are naturally resistant to root rot, and breed them.

He’s not making genetically engineered Christmas trees, however--at least not yet. Frampton’s lab and other researchers are trying to determine the genetic sequences that code for ideal traits, from cone shape to root-rot resistance, and find markers for these sequences so they can be further studied.

“We are doing DNA sequencing to understand the DNA of Christmas trees, and in the long term, this may lead in the future to genetic engineering,” Frampton said. “But there is still more knowledge and techniques we need to develop before we’re to the point that agriculture is now.”



That’s partly because funding for genetic engineering of plants mostly focuses on the kind we eat, he noted. But it’s also because breeding trees is difficult. Mostly it’s because they’re trees, and, well, trees don’t grow like weeds.

“To see how well it grows, you need to wait six or eight years to see if it’s the tree you want,” Frampton said. “Once we find good trees, we want to cross them, but it takes firs 10 or 12 years before they become reproductively mature, before we can start making crosses among them. So the breeding approach is certainly long-term for improving Fraser firs for Christmas trees.”

The trees are now big business in North Carolina, which is home to 2,500 tree growers producing 5 to 6 million trees a year. Fraser firs are native to Appalachia, especially the craggy peaks of western North Carolina above 3,000 feet in elevation. This chilly, rainy environment may have something to do with the quick closure of the trees’ stomata, small respiratory holes on the needles, once they’re cut. Fraser firs adapted to live in very wet environments, and they don’t dry out like other firs and pines--another key feature that makes them ideal Christmas trees.

But still, every tree is unique, both in its individual history and circumstances. My tree, for instance, might have gone without water for weeks after it was cut, or maybe it lived through warm winters, which could have impacted the way its needles set. Or maybe it's just genetics. Frampton said he hopes future trees will be more reliable.

“That’s why we are looking at needle retention and trying to understand how we can manipulate it through genetics,” he said. “But we are also trying to understand how we can handle trees better, and teach consumers best practices to reduce the likelihood that they will have a messy Christmas.”


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Cancer Cells Can Be Killed by Blood-Dwelling Jellyfish

11/15/2012

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Tentacles versus blood cells
Microbiology is really turning into the answer to pretty much every health problem. Even promising cancer cures are being based on it.

What can be seen in that photo above isn't a cure per se, though. Instead, it is more of an agent that can eliminate cancer cells.

Basically, it is a microfluidic chip coated with long strands of DNA which, like a jellyfish scoops grub in the ocean, uses the strands to dangle into the bloodstream and pluck cancerous proteins out.

Designers from Boston's Brigham and Women's Hospital are behind the creation of this chip. They believe that the “jellyfish” can both diagnose and treat the disease. Human testing is the only step left before establishing whether or not the method is worthwhile.

It might be too much to hope for, but it really was about time someone found out how cancer can finally be cured completely.

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Drug Found to Shrink Brain Tumors in Tuberous Sclerosis Complex Patients

11/15/2012

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Drug used to prevent organ rejection can be used to shrink TSC-related tumors
According to a new study only recently made public, a drug (i.e. everolimus) that was initially designed to keep an individual's body from rejecting transplanted organs can also be used to shrink the brain tumors developed by patients suffering with the Tuberous Sclerosis Complex (TSC). 

Dr. Franz, presently working with the TSC Clinic at Cincinnati Children's Hospital Medical Center, explains that, after just 42 weeks of being administered this drug, 35% of the patients who took part in this research witnessed their brain tumors being reduced to nearly half of their original size. 


“Every patient in this study experienced a decrease in size of their tumors, and no patient required surgery for their tumors after treatment with everolimus,” Dr. Franz said. 

Prior to this drug's being made available as a treatment alternative, most of the children and adolescents who developed said type of brain tumors had to undergo surgery in order to gain a better quality of life. 

However, thanks to this drug's being supported by the US Food and Drug Administration, “Children and teens may not only avoid surgery but they also may see improvement in other aspects of this disease,” Dr. Franz believes. 

In other words, the presence of everolimus in one's body inhibits and/or slows the cell growth typically associated with TSC. 

As well as this, it was found to prevent or eliminate altogether the buildup of fluid inside the skulls of patients who develop this type of brain tumors. 

Interestingly enough, there are some who claim that, because TSC and medical conditions such as Alzheimer’s, type 2 diabetes, Parkinson’s disease, Huntington’s disease and autism share the mTOR pathway, everolimus could successfully be used to treat these other disorders as well, simply because it acts as an mTOR inhibitor. 

Given the fact that, for the time being, about 50,000 people in the US and 1 million individuals worldwide are suffering with TSC, this comes as good news indeed.

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How the Brain Falls Prey to Alzheimer’s Disease

7/15/2012

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First-ever timeline details the evolution of Alzheimer's disease over the years
Scientists with the Dominantly Inherited Alzheimer’s Network (DIAN) international research partnership say that they were recently able to develop the first clear timeline detailing how the brain develops Alzheimer’s disease. 

The new dataset will come in handy for researchers who are working hard towards finding ways of addressing the condition. Alzheimer’s is a neurodegenerative form of dementia that currently has no cure. Its primary mode of action is by damaging neurons and attacking cognitive capabilities. 

Since it primarily manifests itself in the elderly, and the general population of the developed world is growing, the condition is expected to put huge strains on national healthcare systems over the coming decades, PsychCentral reports. 


While the therapies experts managed to propose thus far have largely proven ineffectively at treating the condition, some have argued that this is because the dementia starts manifesting clear symptoms only after it has already taken a hold of the brain.

But the team behind the new dataset, which also included scientists from the University of Washington in St. Louis (WUSL) School of Medicine (WUSM), suggests that the earliest signs of the condition set in as many as 25 years before the first discernible symptoms appear. 

In order to compare the new timeline, the investigators looked at a series of markers for Alzheimer’s disease that appear long before the condition sets in. This was made possible by surveying 128 test subjects who came from families whose genetic history predisposed them to developing the disease.

“A series of changes begins in the brain decades before the symptoms of Alzheimer’s disease are noticed by patients or families, and this cascade of events may provide a timeline for symptomatic onset,” WUSM expert and lead study author, Randall Bateman, MD, says.

“Family members without the Alzheimer’s mutations have no detected change in the markers we tested. It’s striking how normal the Alzheimer’s markers are in family members without a mutation,” he goes on to say. 

The research was made possible by funds provided through the US National Institutes of Health (NIH). Details of the work were published in the latest issue of the prestigious New England Journal of Medicine.

“As we learn more about the origins of Alzheimer’s to plan preventive treatments, this Alzheimer’s timeline will be invaluable for successful drug trials,” Bateman concludes.


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New Cell Delivery Technologies in the Works

7/15/2012

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This image shows microbeads developed by SpherIngenics for cell delivery within the human body
A startup from the Georgia Institute of Technology (Georgia Tech) has recently secured funding from the US Department of Defense (DOD), for the development of new technologies related to delivering cells to any location within the human body. 

Cell delivery is a critical step in the process of repairing damaged tissues. However, the main issue with putting new cells in the body is that the environment they encounter once they reach the bloodstream is extremely hostile. 

Any new structures inserted into the body are immediately attacked and disintegrated by the immune system. This leads to significant inflammation, a condition that poses its own set of problems. If the therapeutic cells are not destroyed by this response, they are at least scattered in all directions.


This means that the impact they were supposed to have on a particular area will be severely diminished. In most cases, the cell injections end up having no effect, but producing multiple side-effects. The new startup, called SpherIngenics, was created as a method of preventing this from happening. 

In order to do this, the company is using technology developed in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech, and at the Emory University. Their method is safe, reliable, yields no significant side-effects, and is entirely repeatable.

In addition to protecting the newly introduced cells from an untimely death, they also prevent them from migrating to other locations in the body, increasing the efficiency of cell delivery therapies by a wide margin. SpherIngenics hopes to capitalize on this approach by creating new protective capsules.

Its efforts are being supported by a two-year, $730,000 Phase II Small Business Innovation Research (SBIR) grant from the DOD. The company was funded by Coulter Department professors Franklin Bost (also the company's CEO), Barbara Boyan and Zvi Schwartz.

“When damaged tissue is being repaired by a cell-based therapy, our microbead technology ensures that cells travel to and remain in the targeted area while maintaining continued viability,” Bost explains.

“This technology has the potential to reduce the cost of treatment by eliminating the need for multiple therapeutic procedures,” the expert goes on to say. SphereIngenics was founded back in 2007.

“For the Phase II SBIR grant, we’re going to examine whether delivering microbeads full of stem cells can enhance cartilage repair and regeneration of craniofacial defects in an animal model,” Boyan adds.


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DIY Natural Solar Cells Use Plant Wastes

3/12/2012

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Andreas Mershin
Andreas Mershin, a researchers at the Massachusetts Institute of Technology, has a big dream, one that could potentially change some parts of the world. He believes that it is possible to create inexpensive solar panels from plant wastes, an inexpensive peptide powder, and an ordinary substrate material. 

The expert says that the molecules plants use to conduct photosynthesis could be removed from plant wastes via a relatively simple process, then stabilized using the peptide powder his team is currently working on developing. 

The gooey mixture will then be placed on a sheet of metal, a piece of glass or other ordinary material. Attaching a couple of wires to the substrate would complete the DIY solar panels. If this approach succeeds, the costs associated with building your own power supplies would be negligible. 

Above is the video the MIT News Office compiled of the researcher explaining his technique in more detail. I do hope this works, since renewable energy would help alleviate a host of problems, especially in the developing and Third worlds. 

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New Pacemaker Model Is Powered by the Heart

3/5/2012

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Pacemakers help the heart beat at a healthy rhythm
Heartbeat vibrations could one day power up a new generation of pacemakers, say scientists at the University of Michigan, in the US. If such a technique could be applied to devices controlling the heart beat, then the need to perform battery replacement surgery would disappear. 

At this time, people who carry pacemakers need to undergo regular surgery, where doctors change the batteries powering up their medical implants. This is very uncomfortable, so scientists have been looking for ways to eliminate this procedure from their work flow for a long time. 

Now, U-M engineering researchers propose a new solution to this problem. They say that each heartbeat reverberates through the chest, producing small vibrations. By using a new device the team developed, it may be possible to convert these vibrations into electricity. 

This energy could then be used to power up pacemakers or an implanted defibrillator. Both these tools are used to force the heart into keeping a healthy rhythm in cardiac patients. In devices implanted today, small batteries provide the needed current.

Each patient with such an implant needs to undergo surgery once every 5 to 10 years, in order to have the battery replaced. The waiting period between surgeries is determined by the volume of work the implant needs to carry out. 

“The idea is to use ambient vibrations that are typically wasted and convert them to electrical energy. If you put your hand on top of your heart, you can feel these vibrations all over your torso,” U-M Department of Aerospace Engineering research fellow Amin Karami explains. 

Funds for this investigation were secured from the US National Institute of Standards and Technology (NIST) and the Virginia Tech Institute for Critical Technology and Applied Science.

The U-M group has not yet built a working prototype. However, it did manage to develop a series of schematics and blueprints for how the vibration-powered device should look like. The most important component is a thin slice of a piezoelectric material.

Piezoelectrics are special ceramics and crystals that produce a small electrical current when a mechanical force is exerted onto them. Heart vibrations can easily distort the shape of the thin piezoelectrics slices that the team wants to use. 

Details of the device are published in a paper called “Powering pacemakers from heartbeat vibrations using linear and nonlinear energy harvesters,” which appears in the current issue of the esteemed journal Applied Physics Letters.
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Arctic Bacteria Underlie New Salmonella Vaccine

3/5/2012

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New, temperature-sensitive vaccine developed against Salmonella enterica
Treating salmonella infections in poultry is rapidly becoming one of the most important methods of increasing food security. Working towards that end, a team of Canadian researchers was recently able to isolate a microorganism in the Arctic that may be of help. 

Investigators at the University of Victoria, led by microbiologist Dr. Francis Nano, believe that bacteria collected from cold Arctic waters may hold the key to immunizing chickens against Salmonella enterica infections.

This bacteria causes millions of deaths annually in official statistics, which means that the actual numbers may be even higher. In Canada, around 10,000 people are infected by the pathogen yearly.

As such, salmonella is viewed as a critical public health concern. Scientists say that it represents the most common, food-borne disease in the world. In addition to being harmful in and of itself, the organism also favors the development of other conditions.

Using funds provided through the Genome BC Proof-of-Concept initiative, Nano and his team have been working on a way to use the Arctic bacteria in a new vaccine against S. enterica. Their approach (simple in theory, but difficult to apply in practice) appears to be working, PhysOrg reports. 

The research resulted in the creation of a temperature-sensitive vaccine. What the chemical does is it replaces one of the genes in S. enterica with a gene from the Arctic bacteria. The switch renders the former unable to resist the temperatures in the bloodstream of warm-blooded animals. 

The modified salmonella will then immediately die out, immunizing the host to future infections by the same strand. The team was able to demonstrate in its investigation that producing the vaccine would also be cost-effective. 

This implies that it could be used by authorities, farmers and big companies alike. All those involved in raising animals would stand to benefit from this substance, as would national economies, experts say.

“Using Arctic genes, we can create bacterial pathogens that behave like vaccines, much like the many temperature-sensitive viruses that are used as vaccines. We can apply this same approach to develop new vaccines against many diseases of humans and animals,” Nano explains. 

Vaccinations are becoming increasingly important in animals nowadays because overuse of antimicrobial drugs and antibiotics has given rise to a wide array of drug-resistant microorganisms, which cannot be killed by conventional means.

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Environment Affects How Superbugs Move Around

1/15/2012

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People moving in and out of an area help superbugs receive fresh biological material that enables them to resist antibiotics.
In a new investigation on the spread and infection patterns displayed by antibiotic resistant bacteria – commonly known as superbugs – researchers discovered that their prevalence was higher in villages located along roads than in settlements that were out of reach, or difficult to get to.

This appears to suggest that roads played a never-before-considered role in helping superbugs such as antibiotic-resistant Escherichia Coli (E. coli) spread throughout the general population. The new findings confirm previous studies that roads favor the spread of diseases. 

However, what the other researches did not evidence was a connection between proximity to a road and the chances a person has of being exposed to antibiotic-resistant bacteria. This study was carried out by researchers at the University of Michigan(U-M) School of Public Health.

Details of the new work were published in a paper called “In-roads to the spread of antibiotic resistance: regional patterns of microbial transmission in northern coastal Ecuador,” which is published in this month's issue of the Journal of the Royal Society, Interface.

U-M professor Hoe Eisenberg was the leader of the research team, which also included colleagues from the Universidad San Francisco de Quito, and Trinity College. He is also a coauthor of the new study. 

The study took experts in the northwestern regions of Ecuador. Over a period of five years, the team carried out tests on available strains of antibiotic-resistant E. coli, using a combination of ampicillin and sulfamethoxazole as treatments. 

“Our results show it's not just the individual's antibiotic use that affects antibiotic resistance,” the U-M investigator explains. He says that roads provide two factors that tip the scales in the bacteria's favor.

“Important factors that affect the spread of antibiotic resistance are the rates at which people introduce new strains due to movement in and out of the region, as well as poor water quality and sanitation that allow for the transmission of antibiotic resistant strains,” he explains.

This is one of the few studies conducted to date that also takes into account the broader environmental and social context governing the development of resistance to antibiotics in bacteria. Thus far, a lot of emphasis was placed on the individual, and the way in which they consume antibiotics.

“If we want to think about how we deal with antibiotic resistance we have got to think about the broader environmental forces that cause the spread of antibiotic resistance, in addition to how doctors prescribe antibiotics to individuals,” Eisenberg explains.

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Roboticians Dream of Microbe-Powered Robots

1/15/2012

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Microbes could be used to extend the lifespan of missions going to other worlds.
Space robotics experts at the US Naval Research Laboratory (NRL) are working on a new generation of exploration rovers. They say that the machines would look entirely different from the robots we're used to seeing launch in space. For starters, they will be powered by microbes.

Scientists acknowledge the fact that future space missions will need to be able to send back vast volumes of data. Until now, that need was covered by space agencies building larger and larger spacecraft. But that path is unsustainable. 

NASA already sent two Mars Exploration Rovers to the Red Planet, and then followed up 8 years later with the Mars Science Laboratory (MSL) rover Curiosity. The latter is so immense that it rivals a small SUV in size, while weighing about a ton. 

In order to send even larger machines to other worlds, engineers would have to build extremely expensive rockets, and also make nuclear energy portable. Another solution, the NRL group believes, is to go small, and obtain vast volumes of data through another approach. 

The team says that a swarm of robots, weighing less than one kilogram each, could fulfill the same tasks as a more complex machine, but at a fraction of the cost, with lower energy consumption, and with greater resilience. 

Investigators with the NRL Spacecraft Engineering Department are working under a NASA Innovative Advanced Concepts (NIAC) research grant to develop small robots that would be able to investigate other planets, all while powered by nothing more than bacteria. Expert Gregory Scott leads the team.

Microbial fuel cells are one of the most promising avenues of research in space exploration today, because they can provide reliable energy for extended periods of time, a lot longer than even the nuclear reactor currently powering the MSL.

The actual power source would be the metabolic processes going on inside the bacteria themselves. The electrons the microorganisms generate are sent through a anode-cathode-resistor circuit, which then generates electricity, Wired reports.

Such a battery would recharge naturally, since microorganisms are capable of reproduction. This approach offers an elegant solution to long-term power storage problems currently plaguing missions destined for the far reaches of the solar system. 

“As we move forward in the utilization of MFC as an energy generation method, this research begins to lay the groundwork for low powered electronics with a long-term potential for space and robotic applications,” Scott said in a recent statement. 

“Microbial fuel cells coupled with extremely low-power electronics and a low energy requirement for mobility addresses gaps in power technology applicable to all robotic systems, especially planetary robotics,” he concluded.

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